Preparation of slides2018-10-05T10:47:19+00:00

Preparation of slides

If your specimens are in the form of fixed or frozen tissues or as paraffin embedded tissue blocks, we cut the sections using various histological techniques while respecting the specificities of your project.

Trimming :

This step consists in taking samples from the fixed specimens (conventionally in formalin) and then including the sections of interest in the appropriate histological cassettes.

Paraffin wax impregnation & Inclusion :

The paraffin wax technique is the most commonly used method for studying tissue morphology. The impregnation protocols validated at HISTALIM are compatible with a majority of staining or labelling methods. The inclusion step is done in compliance with the RITA (Registry of Industrial Toxicology Animal-data) standard.

Microtomy & Cryotomy :

Making paraffin embedded or frozen sections respectively have different advantages.

Paraffin embedded sections make it possible to preserve the tissue morphology better while offering the advantage of simple and stable preservation with time.

Frozen sections are used when one wishes to label soluble nutrients (eg.: mineral salts, polypeptides, etc.) or for labels which target elements sensitive to the effect of formalin (eg.: the epitopes of certain proteins can be revealed by immunohistochemistry only by using frozen sections).

Look at our presentation leaflet on our common techniques in histology

High density tissue section cutting :

We have the ability to make sections of undecalcified tissues which may have extremely high density levels (eg.: tissues with an implanted medical device). The samples are incorporated in semi rigid and rigid soft resin blocks, and then polymerized by photo-control (eg.: Polymethylmethacrylate: PMMA). This technique uses a diamond saw for making sections while maintaining the morphology of the various adjoining structures with precision.

Look at our presentation leaflet on the diamond band saws microtome