The modified Russel-Movat staining highlights a lot of tissue components in histological slides. It is obtained by a mix of five stains: alcian blue, Verhoeff hematoxylin, crocein scarlet combined with acidic fuchsine and saffron. At pH 2.5, alcian blue is fixed by electrostatic binding with the acidic mucopolysaccharides. The Verhoeff hematoxylin has a high affinity for nuclei and elastin fibers, negatively charged. The combination of crocein scarlet with acidic fuchsine stains acidophilic tissue components in red. Then, collagen and reticulin fibers are unstained by a reaction with phosphotungstic acid and stained in yellow by saffron.
Modified Russel-Movat staining is used to study the heart, blood vessels and connective tissues. It can also be used to diagnose vascular and lung diseases.
Modified Russel-Movat staining can be applied to all organs and tissues.