Oncology2018-11-20T14:00:17+00:00

Exemples of achievements

Oncology

Description

Species : mouse

Sample : brain

Staining : hematoxylin eosin

Case study summary

Hematoxylin Eosin staining of glioma in the mouse. Metastases is detected in the area close to the dentate gyrus of the hippocampus.

Description

Species : rat

Sample : brain

Staining : Cresyl violet

Case study summary

Cresyl violet staining in rat brain for characterize gliomas.

Description

Species : mouse/human

Sample : xenograft

Labeling : vimentin

Case study summary

Vimentin staining to study conjonctive tissue (expression in primitive nodes and mestastasis to diagnosis mesenchymal origin of tumor)

 

Description

Species : mouse/human

Sample : xenograft (intestine)

Labeling : Alpha-SMA and CD31

Case study summary

Development of a method of image analysis for characterizing angiogenesis within tumours (α-SMA in red and CD31 in brown).

Description

Species : mouse/human

Sample : xenograft

Labeling : HER2

Case study summary

Determination of the HER-2 status of paraffin embedded xenograft tumours by immunohistochemistry of HER-2 (can also be performed with in situ hybridization).

 

Phospho-EGFR receptor quantification on tumor xenografts

The epithelial growth factor receptor (EGFR) is particularly over expressed in several tumour pathologies (breast tumours, carcinoma, etc.). When it is bound to the epidermal growth factor (EGF), it is partly responsible for cell division. It is therefore a signalling pathway very much exploited in the research for novel therapies.

We have developed an application for image analysis which aims to quantify the expression of phospho-EGFR. In this video, we show you how to quantify this receptor in a xenograft model and measure its expression level. One can thus obtain indications regarding the efficacy of the treatment tested.

Species : Human/Mouse
Tissue : Xenograft