Cosmetic-dermatology2018-11-20T14:00:16+00:00

Exemples of achievements

COSMETIC / DERMATOLOGY

Description

Species : mouse

Sample : skin

Staining : hematoxylin eosin

Case study summary

Study of wound healing by measuring the granular layer and filling of the wound in a mouse model of diabetes.

Description

Species : mouse

Sample : skin

Staining : Masson’s trichrome (green light)

Case study summary

Masson’s trichrome stain cell nodes in blue/black, cytoplasmic aereas in pink, erythrocytes and keratin in red, collagen fibers in green. It can be used on human skin, reconstructed epidermes or skin explants to validate the compound efficacity.

Description

Species : human

Sample : skin

Staining : Fontana Masson’s staining

Case study summary

Histological assesment of a pro/anti-pigmenting effect by melanin quantification.

Description

Species : mouse

Sample : skin

Staining : hematoxylin/eosin/saffron staining

Case study summary

HES staining can be used to characterize a UV protector efficiency for example.

Description

Species : human

Sample : skin

Labeling : collagen IV

Case study summary

Collagen IV is a structural protein of the skin. Its evaluation makes it possible to determine the degree of elasticity which is directly related to the state of aging of the skin.

 

Description

Species : human

Sample : skin

Labeling : CD31

Case study summary

Mainly used for demonstrating the presence of endothelial cells, it makes it possible to determine the extent of angiogenesis for example.

 

Description

Species : human

Sample : skin

Labeling : Melan-A

Case study summary

Melan-A is a protein expressed by melanomas and by benign adult melanocyte cells. This labelling is used for quantifying the production of melanin.

 

Description

Species : human

Sample : skin

Labeling : KI67

Case study summary

Ki67 immunolabeling assesment of epiderm mitotic index.

Evaluation of skin regeneration thickness analysis

The European regulations for the marketing of a product for cosmetic and dermatological use requires tests for the efficacy and safety of each new compound. HISTALIM offers image analysis tools making it possible to validate easily and reliably, all types of claims.

In this video, we present a method for quantifying tissue regeneration after lesion by measuring the thickness of the epidermal layer.

Species : mouse

Sample : skin

Staining : Hematoxylin Eosin