Exemples of achievements
Species : human
Sample : liver
Staining : sirius red
Case study summary
Classification of the various levels of liver fibrosis in terms of the quantity of collagen (in red). Related paper :
Automatic scale-independent morphology-based quantification of liver fibrosisJ.Coatelen et al. SPIE Medical Imaging, March 2014
Hepatic fibrosis, collagen quantification on human liver
Liver fibrosis is formed following a chronic localized inflammation which replaces the supporting tissue with fibrous and dense plaques. In an advanced stage such as cirrhosis, the exchanges between the hepatocytes and the blood circulation are reduced tending towards liver failure. HISTALIM has designed a tool to identify the fibrotic areas using collagen labelling. Following image analysis, it makes it possible to quantify the collagen and to objectively determine a classification of the level of fibrosis.
Species : Human Tissue : Liver Staining : Sirius red
Atherosclerosis, spatial distribution of lipids and collagen
Atherosclerosis is a form of arteriosclerosis which is characterized by the formation of atheromatous plaques in the inner layer (intima) of the arteries. The accumulation of cells, lipids and other debris in the arteries can lead to thrombosis which could cause embolism (principal cause of myocardial infarction or CVA).
HISTALIM has developed and validated a method of image analysis making it possible to characterize the spatial distribution of lipids and collagen in the atheromatous plaques. We present, in this video, the results obtained in a section of the mouse aorta as part of an efficacy test of a therapeutic molecule.
Species : Mouse Tissue : Aorta Staining : Sirius red, O red Oil, hematoxylin eosin Labeling : F4/80