LaBiotech Tour France

LaBiotech Tour France is the first ever made documentary film about French Biotech ecosystem. Over 5 weeks, Philip and Joachim biked around France to meet 26 different companies and interview senior leadership. It has then been shown around Europe (Paris, Berlin, London, Copenhagen) and in the United States (NIH-Washington DC, Boston-Merck and San Francisco & San Diego-BIO by July).


HISTALIM, Toxicology : Evaluation of cross-reactivity on human Tissue MicroArray (TMA)

The studies of Tissue Cross Reactivity (TCR) make it possible to assess the cross-reactivity of antibodies in human or animal tissues. This type of test is used prior to toxicity tests for therapeutic antibodies. In this video, we present the evaluation of cross-reactivity through TCR in frozen human Tissue Micro Array (TMA).

Paired with an image analysis tool, our quantitative results are free of any operator effect. This solution provides the opportunity of screening a compound quickly and inexpensively, during early evaluation of toxicity.


Chronic diseases

Colitis, ALU probe/ TNF-alpha colocalization

Ulcerative colitis is a superficial disease of the mucous membrane of the colon and the rectum characterized by the inflammation and formation of lesions in tissue walls. HISTALIM has designed a method making it possible to co-localize 2 relevant markers for this humanized mouse model of colitis:

  • Immunolabelling of TNF-alpha, which is a growth factor playing a role in the inflammatory reaction
  • In situ hybridization with the ALU probe for characterizing human immune cell infiltration

Together, these two markers make it possible to test the efficacy of therapies on Crohn type lesions, for example.

In collaboration with TransCure bioServices.

Non health sector

Image analysis for muscle fiber structure assessment

The microstructure of foodstuffs has a direct impact on the organoleptic properties. At HISTALIM, we have developed a histological method for quantifying the morphological changes of the muscular fibres (Meat Destructuration Indicator: MDI) enabling the classification of meat and bone raw materials. This image analysis tool is the only COFRAC accredited quantitative histological method of analysis in Europe.


Evaluation of skin regeneration thickness analysis

The European regulations for the marketing of a product for cosmetic and dermatological use requires tests for the efficacy and safety of each new compound. HISTALIM offers image analysis tools making it possible to validate easily and reliably, all types of claims. In this video, we present a method for quantifying tissue regeneration after lesion by measuring the thickness of the epidermal layer.

Chronic diseases

Atherosclerosis, spatial distribution of lipids and collagen

Atherosclerosis is a form of arteriosclerosis which is characterized by the formation of atheromatous plaques in the inner layer (intima) of the arteries. The accumulation of cells, lipids and other debris in the arteries can lead to thrombosis which could cause embolism (principal cause of myocardial infarction or CVA).

HISTALIM has developed and validated a method of image analysis making it possible to characterize the spatial distribution of lipids and collagen in the atheromatous plaques. We present, in this video, the results obtained in a section of the mouse aorta as part of an efficacy test of a therapeutic molecule.


Phospho-EGFR receptor quantification on tumor xenografts

The epithelial growth factor receptor (EGFR) is particularly over expressed in several tumour pathologies (breast tumours, carcinoma, etc.). When it is bound to the epidermal growth factor (EGF), it is partly responsible for cell division. It is therefore a signalling pathway very much exploited in the research

for novel therapies. We have developed an application for image analysis which aims to quantify the expression of phospho-EGFR. In this video, we show you how to quantify this receptor in a xenograft model and measure its expression level. One can thus obtain indications regarding the efficacy of the treatment tested.


Hepatic fibrosis, collagen quantification on human liver

Liver fibrosis is formed following a chronic localized inflammation which replaces the supporting tissue with fibrous and dense plaques.  In an advanced stage such as cirrhosis, the exchanges between the hepatocytes and the blood circulation are reduced tending towards liver failure. HISTALIM has designed a tool to identify the fibrotic areas using collagen labelling. Following image analysis, it makes it possible to quantify the collagen and to objectively determine a classification of the level of fibrosis.

Non-health sector

Non-Health sector: Assessment of GonadoSomatic Index (GSI) in oyster

The determination of the gonadosomatic ratio (percentage of the body volume of the animal occupied by the gonad) makes it possible to follow the smooth continuity of reproduction in molluscs in livestock farming. It is also used in research for selecting the oysters which spend their energy in growth rather than in reproduction, thus making it possible to obtain oysters of larger size more quickly. It is also important to know if the oysters selected for their growth can or cannot reproduce in natural environment.

Other diseases

other diseases : Wilson’s disease, quantification of copper deposition in the liver

WILSON’s disease is a genetic disorder which is characterized by a toxic accumulation of copper in the organism. The lesions are essentially localized in the liver and the brain.

In this video, we present a technique for the analysis of images, developed internally by HISTALIM, making it possible to highlight and quantify the copper deposits in a mouse liver (pathological model). This tool may be applied in toxicology, preclinical development and more specifically in the context of WILSON’s disease.