Immunohistochemistry :
What is IHC ?
The aim of immunohistochemistry (IHC) is the detection of proteins and their cellular localisation in tissue sections that have been fixated and embedded in paraffin. The protein is first detected by labelled antibodies and then shown with a colorimetric reaction. Alternatively fluorescence can be used.
Applications
Immunohistochemistry (IHC) is commonly used for the diagnosis and monitoring of cancers and other pathologies. It can also be used to identify cellular or tissue types, in the neurosciences to study protein expression of the various cerebral structures or in veterinary histopathology.
Critical control point
The quality of results of immunohistochemistry depends on numerous variability factors, mainly sample fixation and embedding conditions, operator variability, potential epitope retrieval steps, or antibody storage conditions.
To ensure the reproducibility and reliability of results, it is therefore necessary to control all of the critical points of the protocol.
HISTALIM is equipped with state of the art machines that can manage and optimize all of the IHC stages, from the receiving of fresh tissue to the interpretation, guaranteeing the solidity of the procedure and a high level of result reproducibility.