HISTALIM, Toxicology : Evaluation of cross-reactivity on human Tissue MicroArray (TMA)
The studies of Tissue Cross Reactivity (TCR) make it possible to assess the cross-reactivity of antibodies in human or animal tissues. This type of test is used prior to toxicity tests for therapeutic antibodies. In this video, we present the evaluation of cross-reactivity through TCR in frozen human Tissue Micro Array (TMA).
Paired with an image analysis tool, our quantitative results are free of any operator effect. This solution provides the opportunity of screening a compound quickly and inexpensively, during early evaluation of toxicity.
- Immunolabelling of TNF-alpha, which is a growth factor playing a role in the inflammatory reaction
- In situ hybridization with the ALU probe for characterizing human immune cell infiltration
Together, these two markers make it possible to test the efficacy of therapies on Crohn type lesions, for example.
In collaboration with TransCure bioServices.
HISTALIM has developed and validated a method of image analysis making it possible to characterize the spatial distribution of lipids and collagen in the atheromatous plaques. We present, in this video, the results obtained in a section of the mouse aorta as part of an efficacy test of a therapeutic molecule.
We have developed an application for image analysis which aims to quantify the expression of phospho-EGFR. In this video, we show you how to quantify this receptor in a xenograft model and measure its expression level. One can thus obtain indications regarding the efficacy of the treatment tested.
In this video, we present a technique for the analysis of images, developed internally by HISTALIM, making it possible to highlight and quantify the copper deposits in a mouse liver (pathological model). This tool may be applied in toxicology, preclinical development and more specifically in the context of WILSON’s disease.